Coding

Part:BBa_K1954006:Design

Designed by: Kamil Żmijewski   Group: iGEM16_UCL   (2016-10-19)


Feedback-resistant N-acetylglutamate synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 417
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 417
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 417
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 417
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 682


Design Notes

One restriction site was removed from the source sequence by silent mutagenesis as follows:

PstI 421: Leu 121 (CTG) 46.9 % -> Leu (TTA) 15.2 %


Source

AF008119.1

References